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. 2011 Nov 8;31(2):471–480. doi: 10.1038/emboj.2011.402

Figure 2.

Figure 2

Inhibition of HCT116 cell proliferation after ASPP1/YAP down-regulation is p53 and p21 dependent. (A) Cell proliferation of HCT116 cells transfected with control or ASPP1 siRNA was quantified by Trypan blue exclusion. (B) Cells transfected as in (A) were synchronized at the G1/S-phase transition by double thymidine block, and analysed for BrdU incorporation at the indicated times following release. (C) Cell cycle of HCT116 transfected by the indicated siRNA was analysed by BrdU and PI incorporation, measured by flow cytometry. Results represent the mean of three independent experiments. (D) p53−/− HCT116 cells were transfected and analysed as in (C). Results represent the mean of three independent experiments. (E) HCT116 cells transfected with siRNA targeting p21 (LHS), or p21−/− cells (RHS) were treated and analysed as in (C). Results represent the mean of three independent experiments. Note the control siRNA data in (E) are the same as those shown in (C).