Table 1. Summary of the ITC data.
Complex | Kd (nM) | ΔH (kcal mol−1) | TΔS (kcal mol−1) | ΔG (kcal mol−1) | n |
---|---|---|---|---|---|
Pex415−183:Pex22S | 2.0±0.08 | −18.2±1.6 | −6.3±1.6 | −11.9±0.1 | 1.16±0.01 |
Ubc4p:Pex22S | No binding detectable | ||||
Pex415−183 Y172A:Pex22S | No binding detectable | ||||
Pex415−183 Y172F:Pex22S | 25.2±2.8 | −6.4±0.9 | 3.9±0.6 | −10.4±0.06 | 1.13±0.03 |
Pex415−183:Pex22S K133A | 18.4±5.0 | −6.9±0.9 | 3.7±1.1 | −10.6±0.1 | 0.92±0.05 |