Transgressive PAL siRNA accumulate in IL8-3. (A) Heatmap showing changes in the level of sRNA from the most transgressive cDNA loci in the IL samples. The major families of transgressive siRNAs correspond to the following genetic loci: PAL, phenylalanine ammonia–lyase; EMB, Embryo defective; HB5,Homeobox protein family; VTC, ‘Vitamin C deficient’ family; WIP, Wound inducible protein family. Transgressive siRNA loci are listed in Supplementary Table SIII. (B) sRNAs aligning to the PAL locus in M82, S. pennellii, F1, F2 and ILs. Arrows indicate direction of sRNA alignment, and the colour of the arrow indicates length: blue, 24-nt; red, 21-nt; and green, 22-nt. (C) Northern blot analysis of small RNAs hybridising to a PAL-specific probe.