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. 2011 Aug;59(8):727–740. doi: 10.1369/0022155411410885

Figure 2.

Figure 2.

Cy5–small interfering RNA (siRNA) biodistribution in mouse liver and in spleen. Sections were stained with phalloidin (green) and counterstained with DAPI (blue). (A–D) Cy5–siRNA distributed in the liver is time dependent. (A) 30 min, (B) 2 hr, (C) 24 hr, and (D) PBS control. (E–H) Overlaid images of Cy5 (purple) and phalloidin outlined liver cells (green) and nuclei (DAPI blue) for the time points corresponding to A–D. Abundant Cy5 signals detected in the sinusoids of liver; diffuse Cy5 siRNA signals detected in liver parenchyma starting at 30 min post dose, lasting at least 24 hr. (I–L) Cy5–siRNA detected in the red pulp of spleen at 30 min (I), 2 hr (J), and 24 hr (K); PBS control in (L). V for central veins or portal veins of the liver. W for white pulp and R for red pulp of the spleen.