Figure 3.

Cy5–small interfering RNA (siRNA) biodistribution in different lobes of mouse livers: Four slices of liver from caudate, left, right, and medial lobes are illustrated in A, which is stained with phalloidin (green) and DAPI (blue). LNP201–siRNA–Cy5 was dosed for 0.5 hr (D, E) and 2 hr (A, B, C). Enlarged images from medial lobe were illustrated in B and C. At the same scale, images from a medial lobe of 0.5 hr dosing group were illustrated in D and E. Cy5–siRNA is purple in B and D. Overlaid images of Cy5, phalloidin (green), and DAPI nuclei (blue) showed in C and E. Cy5–siRNA signals relatively evenly distributed in the entire liver and no Cy5 signals inside the veins. Arrow heads indicate zone I, portal triad areas. Arrow indicates zone III, central vein area. Zone II is the area between I and III.