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. 2011 Nov;59(11):1009–1021. doi: 10.1369/0022155411423406

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Figure 1. — (A) Dentin sialophosphoprotein (DSPP) mRNA in different tissues. The expression of DSPP and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) control was examined by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis using RNA extracted from the tissues of 1-month-old mice. Lane 1, teeth (incisor); lane 2, bone; lane 3, salivary glands; lane 4, articular cartilage from mandibular condyle; lane 5, liver; lane 6, kidney; lane 7, brain; lane 8, heart; lane 9, spleen. Note that positive results were observed in the salivary glands, cartilage, liver, kidney, and brain but not in the heart and spleen. GAPDH control shows similar positive result for all samples. (B) Relative expression level of DSPP mRNA in non-mineralized tissues. The mRNA level of teeth was set at 100% for real-time PCR calculations. The level of DSPP mRNA in other tissues was then calculated relative to the level found in the teeth. Note that the expression levels of DSPP in salivary glands, articular cartilage, liver, and kidney are higher than that of bone.