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. Author manuscript; available in PMC: 2012 Jan 19.
Published in final edited form as: Glycoconj J. 2009 Oct 15;27(2):227–236. doi: 10.1007/s10719-009-9262-9

Figure 1.

Figure 1

Immunoreactivity of the 9C4 mAb with intact galectin-3 but not with the galectin-3 CRD. Biotinylated, recombinant galectin-3 was incubated with C. histolyticum collagenase and subjected to electroblot analysis. Streptavidin identified biotinylated galectin-3 (~30 kDa) in the untreated and treated samples and the biotinylated, cleaved galectin-3 CRD (~17 kDa) in the treated sample. The 9C4 mAb reacted with intact galectin-3 (~30 kDa) in the untreated and treated samples and with a protein band of the molecular mass (~10 kDa) anticipated for the 107 amino acid, N-terminal galectin-3 protein fragment generated by collagenase Type VII cleavage. 9C4 immunoreactivity with the CRD was not apparent. Molecular weight markers are indicated in kDa.