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. Author manuscript; available in PMC: 2012 Jan 19.
Published in final edited form as: Glycoconj J. 2009 Oct 15;27(2):227–236. doi: 10.1007/s10719-009-9262-9

Figure 3.

Figure 3

Differential ultracentrifugation analysis of galectin-3 in seminal plasma. Clarified seminal plasma was ultracentrifuged to separate the soluble fraction (supernatant) from the membrane fraction (pellet). The membrane fraction was treated with 0.5% Triton X-100 and ultracentrifuged to separate the detergent-soluble (supernatant) and detergent-insoluble (pellet) membrane fractions. Twenty μg (13.1 μl) clarified seminal plasma and 13.1 μl soluble fraction, detergent-soluble membrane fraction, and detergent-insoluble membrane fraction were evaluated by immunoblot analysis with the anti-galectin-3 mAb to compare the relative amount of galectin-3 in each fraction.