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Prostasome purification by size exclusion chromatography and galectin-3 immunoblot analysis. The membrane fraction from seminal plasma was subjected to size exclusion column chromatography on Sephacryl S300 and prostasomes were collected in the void volume (fractions 14–19). Aliquots of column fractions were separated by SDS-PAGE. Electroblots were stained for total protein with Ponceau S and for galectin-3 and CD26 immunoreactivity. Molecular weight markers are indicated in kDa.
