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. Author manuscript; available in PMC: 2012 Jan 19.
Published in final edited form as: Glycoconj J. 2009 Oct 15;27(2):227–236. doi: 10.1007/s10719-009-9262-9

Figure 7.

Figure 7

Purification of β-galactoside-binding proteins from the prostasome-enriched membrane fraction. The seminal plasma membrane fraction was extracted to release proteins from the cholesterol-rich prostasome membrane. Extracted proteins were subjected to affinity column chromatography on immobilized lactose. Aliquots of starting material, the final column wash, and eluted fractions containing β-galactoside-binding proteins were separated by SDS-PAGE. Total protein was identified by silver staining (A), and galectin-3 was identified by immunoblot analysis (B). Molecular weight markers are indicated in kDa.