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. Author manuscript; available in PMC: 2012 Jan 19.
Published in final edited form as: Glycoconj J. 2009 Oct 15;27(2):227–236. doi: 10.1007/s10719-009-9262-9

Figure 8.

Figure 8

Sequence coverage of galectin-3 peptides identified by tandem mass spectrometry. The ~30 and ~15 kDa β-galactoside-binding proteins from the seminal plasma membrane fraction were digested with trypsin and analyzed by tandem mass spectrometry. (A) Analysis of the ~30 kDa protein yielded ten peptides (Bold Underline) that matched with the reported amino acid sequence of human galectin-3 (Mascot score = 565) and represented 40% of the galectin-3 sequence. (B) Analysis of the ~15 kDa protein yielded nine peptides (Bold Underline) that matched with the reported amino acid sequence of human galectin-3 (Mascot score = 427) and represented 31% of the galectin-3 sequence.