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. Author manuscript; available in PMC: 2012 Jan 19.
Published in final edited form as: Glycoconj J. 2009 Oct 15;27(2):227–236. doi: 10.1007/s10719-009-9262-9

Figure 9.

Figure 9

Purification of β-galactoside-binding proteins from purified, surface-biotinylated prostasomes. The surface of isolated prostasomes was biotinylated with membrane-impermeable sulfo-NHS-LC-biotin. β-galactoside-binding proteins were affinity purified on immobilized lactose. Non-bound material in the column flow through and purified β-galactoside-binding proteins were subjected to electroblot analysis to identify biotinylated proteins with HRP-conjugated streptavidin and to identify galectin-3 immunoreactivity. Molecular weight markers are indicated in kDa.