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. Author manuscript; available in PMC: 2012 May 13.
Published in final edited form as: Cell. 2011 May 13;145(4):529–542. doi: 10.1016/j.cell.2011.03.041

Figure 2. Inhibition of MRE11 Alleviates Nucleolytic Degradation of Stalled Forks.

Figure 2

(A–B) Preformed IdU tract lengths in V-C8 (A) and V-C8+BRCA2 (B) cells during different exposure times to HU. Inset, the rate of IdU tract length change is 0.7 µm/h, estimated to be ~1.8 kb/h.

(C) Sketch of design and expected outcome of nuclease directionality test. Tick marks delineate lagging strands.

(D–E) Distribution curves of the ratio of CldU/IdU tract lengths with or without HU in V-C8 (D) and V-C8+BRCA2 (E) cells.

(F) IdU tract lengths in V-C8 and V-C8+BRCA2 cells with or without HU in the presence of the MRE11 inhibitor mirin.

(E) IdU tract lengths in Brca2lex1/lex2 mES cells after shRNA treatment directed against MRE11 or control (shLuc) with or without HU. Western blot inset shows the MRE11 knockdown.