Figure 4. BRCA2 Domain Analysis Reveals Differences in Fork Stability and HDR.
(A) Graphical sketch of human BRCA2, PIR2, and BRC3-RPA. PALB2-interaction site (bright green), BRC repeats (dark green bars), DNA binding domain (DBD, black bar), C-terminal region (C-ter) with non-BRC RAD51 binding site (red bar). Arrows indicate truncations in V-C8 cells.
(B–C) IdU tracts in V-C8 cells stably expressing the PIR2 (B) or BRC3-RPA (C) peptides with or without HU.