Skip to main content
. Author manuscript; available in PMC: 2013 Jan 10.
Published in final edited form as: Biochemistry. 2011 Dec 16;51(1):370–381. doi: 10.1021/bi2013905

Figure 2.

Figure 2

DNA decatenation activities of wild-type, GrlAS81F, and GrlAS81Y topoisomerase IV. The ability of wild-type (WT, black squares), GrlAS81F (S81F, red circles), and GrlAS81Y (S81Y, blue triangles) topoisomerase IV to decatenate kinetoplast DNA is shown. Error bars represent the standard deviation of three or more independent experiments. The inset shows an agarose gel of a typical 30 min decatenation time course catalyzed by wild-type topoisomerase IV. The positions of kDNA (K) and monomeric free circles (FC) resulting from decatenation of kDNA are indicated.