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. Author manuscript; available in PMC: 2013 Jan 10.
Published in final edited form as: Biochemistry. 2011 Dec 16;51(1):370–381. doi: 10.1021/bi2013905

Figure 8.

Figure 8

Effects of quinolones and 8-methyl-quinazoline-2,4-dione on sites of DNA cleavage mediated by wild-type, GrlAS81F, and GrlAS81Y topoisomerase IV. An autoradiogram of a polyacrylamide gel identifying DNA sites cleaved by the wild-type (WT), GrlAS81F (S81F), and GrlAS81Y (S81Y) enzymes is shown. Reactions contained no enzyme (No topo), or topoisomerase IV in the presence of the indicated concentrations of Mg2+, Ca2+, or ciprofloxacin (Cipro), levofloxacin (Levo), CP-115,953 (953), or 8-methyl-quinazoline-2,4-dione (Dione). Mg2+ (10 mM) was used in all drug-containing reactions. The autoradiogram is representative of three or more independent experiments.