FIG. 6.

Lateral traumatic brain injury (TBI) caused time- and region-dependent changes in the proportion of Ser3-phosphorylated cofilin (pSer3-cofilin). To determine whether TBI affected the proportion of Ser3 phosphorylated cofilin, pSer3-cofilin immunoreactivity data were normalized to total cofilin immunoreactivity data for each sample, yielding a ratio of pSer3-cofilin to total cofilin (p/t cofilin). Analysis of these data identified time- and region-dependent changes in p/t cofilin. In the ipsilateral hippocampus, for example, p/t cofilin was significantly below control levels at 1 h, 12 h, and 24 h post-TBI, and again at 4 weeks post-TBI (A). In the contralateral hippocampus, a similar reduction in p/t cofilin was observed at 1 h, 12 h, and 24 h post-TBI, and then at 2 weeks post-TBI, relative to controls (B). The ipsilateral neocortex showed an increase in p/t cofilin at 1 h post-TBI, followed by decreases in p/t cofilin at 12 h, 24 h, 2 weeks, and 4 weeks post-TBI, relative to controls (C). A similar pattern was observed in the contralateral neocortex, where p/t cofilin was significantly above control levels at 1 h post-TBI, but then dropped below control levels at 24 h, 1 week, 2 weeks, and 4 weeks post-TBI (D). All comparisons were made by one-way analysis of variance with Dunnett's post-hoc test (*p<0.05, **p<0.01).