Figure 4.

Inhibitory effects of DWE on HIV-1 and another retrovirus. Figure A and B shows the effects of DWE on HIV-1 and another retrovirus pLNCX2 -EGFP replication. Gene expression of NIH/3T3 cells were infected with two pseudotyped retroviruses for 12 h and treated with different concentrations of DWE. Different concentrations of AZT (from 0 μM to 10 μM) and aqueous Herba Artemisiae Scopariae extract (HASWE) were used as positive control and negative control respectively. (A) NIH/3T3 cells infected with VSV-G pseudotyped HIV-1; (B) NIH/3T3 cells infected with MoMuLV/MoMuSV hybrid retrovirus; (C) Percentage of inhibition of HIV-1 RT by DWE. 2.5 μM AZT was used as the positive control with 98% inhibitory rate while lysis buffer with no HIV-1 RT was used as one negative control, lysis buffer with HIV-1 RT but no DWE was used as another negative control. The data represent the mean ± SD of triplicate experiments