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. 2011 Nov 30;18(1):89. doi: 10.1186/1423-0127-18-89

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Copyright ©2011 Drastichova et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunoblot analysis of cytoprotective proteins in myocardial preparations from rats affected by morphine exposure. Samples from control (CON) and morphine-treated (MOR) rats were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with indicated antibodies. All lanes were loaded with 50 μg proteins. The relative levels of ORP150, hypoxia up-regulated protein 1 (A), HSP27, heat shock protein beta-1 (B), HSC70, heat shock cognate 71 kDa protein (C) and GRP78, 78 kDa glucose-regulated protein (C) were assessed by scanning densitometry.