Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Jan 19;7(1):e30101. doi: 10.1371/journal.pone.0030101

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Haeri, Knox.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(a) Representative low magnification CSLM images of a live rod expressing soluble EGFP show the fluorescence distribution (merged with the DIC image, left) and with the region of interest (ROI) indicated (white box). The cell (P, pretreatment) was photobleached in the ROI (0 s) and the recovery was followed for the indicated time (in seconds). (b–f) Live rods expressing Rho-EGFP or Rho^P23H-EGFP were photobleached in different regions and images from different times in the recovery period are shown at higher magnification. The pre-bleach sample is shown as a merge (fluorescence with DIC, left) and with fluorescence alone (P) followed by representative images immediately after bleach (0 s) and 5 s and 115 s post-bleach. The ROI is boxed in each image. Various compartments of the cells were photobleached: (b) base of the OS in cells expressing Rho-EGFP; (c) near the base of the OS in a region with no fluorescent foci in a cell expressing Rho^P23H-EGFP; (d) IS in a cell expressing Rho^P23H-EGFP; (e) in the OS with fluorescent Rho^P23H-EGFP foci; (f) in an axial view of the OS with fluorescent Rho^P23H-EGFP foci. (g) The photobleaching recovery profile of panel (b). Integrated fluorescence of Rho-EGFP in the ROI defined in (b), normalized to the integrated fluorescence of the same region in the pre-photobleached image (closed circles) compared to the fluorescence in a neighboring region (open triangles). (h) Integrated fluorescence recovery after photobleaching in regions of Rho^P23H-EGFP that showed recovery following photobleaching (c and d). Averaged recovery profiles of photobleached areas in the two groups (c and d) were normalized in order to compare the half-time recovery of the mobile fraction of mutant opsin participating in recovery after photobleaching. Rho-EGFP (red trace) is included for comparison. (i) The photobleaching recovery profile of panel (e) from the ROI (closed circles) and a neighboring area (open triangles). There is no significant change in the fluorescence of the foci on the left of photobleaching box during the recovery period (open triangles). Scale bars, 5 µm.