Figure 6. Mast cell–derived mediators in BAL fluid and in vivo assessment of EAR responses with serotonin-mediated bronchoconstriction in the presence of surfactant BAL was performed at 3 minutes after PBS or Ova challenge and mediators were measured by EIA or ELISA kits.

(A) Cysteinyl leukotrienes (n = 5−8/group) and (B) amphiregulin levels (n = 7−9/group) (*p<0.05), (C) Serotonin-induced bronchoconstriction is inhibited by pre-treatment with surfactant, as observed in R_N at the peak response as well as at 5, 10, and 15 minutes after allergen challenge (n = 7, *p<0.05, **p<0.01). (D) At 5 minutes into the response, there is a trend for the inhibition of the G parameter by surfactant (p = 0.08) as well as at 10 minutes (p = 0.065).