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. 2012 Jan 19;7(1):e29677. doi: 10.1371/journal.pone.0029677

Table 2. Oligonucleotide sequences for RT-PCR.

Name Sequence Size (bp) Accession Number
Collagen type IV F: 5′-GGC ACC TGC CAC TAC TAC GC-3′ 472 NM_001845
R: 5′-TCA CCA GGA GGT AGC CGA T-3′
Keratin 12 F: 5′-GAT GCT AAT GCT GAG CTC GA-3′ 393 NM_000223
R: 5′-ACC TGC CCT ACA GCT TTG TA-3
VDAC3 F: 5′-TGA CTC TTG ATA CCA TAT TTG TAC CG-3′ 482 NM_001135694
R: 5′-TCA ATT TGA CTC CTG GTC GAA-3′
CLCN3 F: 5′-AGA AAG GCA TAG ACG GAT CAA-3′ 204 NM_001829
R: 5′-GGT TGT ACC ACA ACG CAC TAA-3′
SLC4A4 F: 5′-GTT CAG ATG AAT GGG GAT ACGC 697 NM_001136260
R: 5′-CGA GCA TAA ACA CAA AGC GTA A-3′
Na+/K+-ATPase F: 5′-CCC AGG ACT CAT GGT TTT TC-3′ 482 NM_000702
R: 5′-GGA GCA AAG CTG ACC TGA AC-3′
N-cadherin F: 5′-CAA CTT GCC AGA AAA CTC CAG G-3′ 205 NM_ 001792
R: 5′-ATG AAA CCG GGC TAT CTG CTC-3′
β-catenin F: 5′-TAC CTC CCA AGT CCT GTA TGA G-3′ 180 NM _001904
R: 5′-TGA GCA GCA TCA AAC TGT GTA G-3′
P-120 F: 5′-CCC CAG GAT CAC AGT CAC CT-3′ 144 NM_001085467
R: 5′-CCG AGT GGT CCC ATC ATC TG-3′
ZO-1 F: 5′-AGT CCC TTA CCT TTC GCC TGA-3′ 180 NM_003257
R: 5′-TCT CTT AGC ATT ATG TGA GCT GC-3′
GAPDH F: 5′-GCT CAG ACA CCA TGG GGA AGG T-3′ 474 NM_002046
R: 5′-GTG GTG CAG GAG GCA TTG CTG A-3′
PCNA F: 5′- GCGTGAACCTCACCAGTATGT-3′ 76 NM_002592
R: 5′- TCTTCGGCCCTTAGTGTAATGAT-3′
CDC2 F: 5′- GGATGTGCTTATGCAGGATTCC-3′ 100 NM_001786
R: 5′- CATGTACTGACCAGGAGGGATAG-3′

VDAC3: voltage-dependent anion channel 3, CLCN3: chloride channel protein 3, SLC4A4: sodium bicarbonate cotransporter membrane.