Table 2. Oligonucleotide sequences for RT-PCR.
Name | Sequence | Size (bp) | Accession Number |
Collagen type IV | F: 5′-GGC ACC TGC CAC TAC TAC GC-3′ | 472 | NM_001845 |
R: 5′-TCA CCA GGA GGT AGC CGA T-3′ | |||
Keratin 12 | F: 5′-GAT GCT AAT GCT GAG CTC GA-3′ | 393 | NM_000223 |
R: 5′-ACC TGC CCT ACA GCT TTG TA-3 | |||
VDAC3 | F: 5′-TGA CTC TTG ATA CCA TAT TTG TAC CG-3′ | 482 | NM_001135694 |
R: 5′-TCA ATT TGA CTC CTG GTC GAA-3′ | |||
CLCN3 | F: 5′-AGA AAG GCA TAG ACG GAT CAA-3′ | 204 | NM_001829 |
R: 5′-GGT TGT ACC ACA ACG CAC TAA-3′ | |||
SLC4A4 | F: 5′-GTT CAG ATG AAT GGG GAT ACGC | 697 | NM_001136260 |
R: 5′-CGA GCA TAA ACA CAA AGC GTA A-3′ | |||
Na+/K+-ATPase | F: 5′-CCC AGG ACT CAT GGT TTT TC-3′ | 482 | NM_000702 |
R: 5′-GGA GCA AAG CTG ACC TGA AC-3′ | |||
N-cadherin | F: 5′-CAA CTT GCC AGA AAA CTC CAG G-3′ | 205 | NM_ 001792 |
R: 5′-ATG AAA CCG GGC TAT CTG CTC-3′ | |||
β-catenin | F: 5′-TAC CTC CCA AGT CCT GTA TGA G-3′ | 180 | NM _001904 |
R: 5′-TGA GCA GCA TCA AAC TGT GTA G-3′ | |||
P-120 | F: 5′-CCC CAG GAT CAC AGT CAC CT-3′ | 144 | NM_001085467 |
R: 5′-CCG AGT GGT CCC ATC ATC TG-3′ | |||
ZO-1 | F: 5′-AGT CCC TTA CCT TTC GCC TGA-3′ | 180 | NM_003257 |
R: 5′-TCT CTT AGC ATT ATG TGA GCT GC-3′ | |||
GAPDH | F: 5′-GCT CAG ACA CCA TGG GGA AGG T-3′ | 474 | NM_002046 |
R: 5′-GTG GTG CAG GAG GCA TTG CTG A-3′ | |||
PCNA | F: 5′- GCGTGAACCTCACCAGTATGT-3′ | 76 | NM_002592 |
R: 5′- TCTTCGGCCCTTAGTGTAATGAT-3′ | |||
CDC2 | F: 5′- GGATGTGCTTATGCAGGATTCC-3′ | 100 | NM_001786 |
R: 5′- CATGTACTGACCAGGAGGGATAG-3′ |
VDAC3: voltage-dependent anion channel 3, CLCN3: chloride channel protein 3, SLC4A4: sodium bicarbonate cotransporter membrane.