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. 2012 Jan 19;7(1):e29514. doi: 10.1371/journal.pone.0029514

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Rahman et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Enzyme activity was determined by measuring the CO produced in 15 min from 50 µM methemalbumin using 0.5 mg/mL rat spleen microsomes. The IC₅₀ was determined by nonlinear regression using GraphPad Prism version 4. Curves represent two independent trials, with each performed in duplicate.