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. 2012 Jan 19;7(1):e29444. doi: 10.1371/journal.pone.0029444

Figure 4. Binding of ligands to SaHPPK as measured by ITC and SPR.

Figure 4

A, Raw (top left) and integrated ITC data (bottom left) for the titration of 30 µM SaHPPK with 300 µM 8-mercaptoguanine (8MG) alone (▪), and 300 µM 8-mercaptoguanine in the presence of 1 mM AMPCPP (•). SPR raw data (middle and right top) and steady-state response curve (middle and right bottom) for the binding of 8-mercaptoguanine in the absence (middle) and presence (right) of 5 mM ATP. B, Raw ITC data (top left) and integrated normalised data (bottom left) for titrations of 22 µM SaHPPK with 500 µM HMDP (▴) in the absence of 8-mercaptoguanine and in the presence of 300 µM 8-mercaptoguanine (•), and in the presence of 1 mM AMPCPP (▪). Binding of HMDP was only detected in the presence of AMPCPP. SPR raw data (middle and right top) and steady-state response curve (middle and right bottom) for the binding of HMDP in the absence or presence of saturated AMPCPP.