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. 2012 Jan 19;8(1):e1002464. doi: 10.1371/journal.pgen.1002464

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Hao et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Intestinal muscle contractions during the defecation motor program (quantified as expulsions/pBoc) were analyzed in the indicated genotypes. (B) Endogenous IPSCs were recorded from adult body wall muscles of the indicated genotypes. Representative traces (left), and summary data (right) are shown. The number of animals analyzed is indicated for each genotype. (C) Representative images (left) and summary data (right) for GFP::SNB-1 (expressed by the unc-25 promoter) in dorsal cord axons of the indicated genotypes. The number of animals analyzed is indicated for each genotype. Values that differ significantly from wild type (**, p<0.001, ***, p<0.0001 Students t-test) and from ric-7 mutants (#, p<0.05, ##, p<0.001, ###, p<0.0001 Students t-test) are indicated. Error bars indicate SEM. For rescue experiments, ric-7 transgenes are as follows: ACh (unc-17 promoter), GABA (unc-47 promoter), pan-neuron (snb-1 promoter), intestine (vha-6 promoter).