Figure 6. Selective advantage of the ‘D67N/K70R/T215Y/K219Q’ mutant against the wild type during RNA- and DNA-dependent polymerization in the presence of AZT-TP.

The solid lines (green = activated cells, blue = unstimulated cells, red = macrophages) indicate the selection parameter , defined in eq. (4), for different levels of intracellular ATZ-TP during RNA- and DNA dependent polymerization (Panels A & B) of a random sequence of length 500 with 25% respective dNTP content. The light grey area indicates the in vivo concentrations range of AZT in purified circulating T-cells from [71], converted to units by assuming a cell volume of for resting T-cells [72]. The dashed horizontal line indicates the threshold for resistance selection, i.e. . All utilized parameters are indicated in Table 1 and Tables S1, S2, S3 (supplementary material).