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. 2011 Aug 22;120(1):62–67. doi: 10.1289/ehp.1103437

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Apoptosis and intracellular ROS. (A,B) Representative dot graphs of unexposed HL-60 cells (control; A) and cells exposed to 50 μM BT for 8 hr (B). Cells in the lower right quadrant, which were stained with annexin V but not PI, were considered to be apoptotic; percentages of these cells in are shown in the figure. (C) Percentages of apoptotic HL-60 cells after exposure to BT for 8 hr; data presented are mean + SE from two independent experiments conducted in duplicate. Inset, percentages of apoptotic cells in controls or HL-60 cells exposed to 50 μM BT for 8 hr (mean + SE of 15 independent experiments conducted in duplicate). (D) Fluorescence intensities, corresponding to levels of various ROS, in controls or cells exposed to 50 μM BT for 30 min (mean + SE from 5–7 independent experiments conducted in duplicate). Fluorescence intensity is shown in arbitrary units. **p < 0.01, and ^#p < 0.001, compared with control.