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Expression of Ahr, Ppara, and metabolism genes in WT and KO mice. (A) qPCR analysis of Ahr mRNA. (B) PPAR-α protein (top; representative immunoblot from three WT and two KO animals) and Ppara mRNA (bottom) in WT and KO mice. (C) qPCR analysis of hepatic β-oxidation and gluconeogenic genes. All samples are from liver of WT (n = 8) and KO (n = 10) mice, collected at ZT8. Data are mean ± SE. *p < 0.05, **p < 0.01, and #p < 0.001 compared with WT mice.
