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. 2011 Aug 3;119(12):1775–1780. doi: 10.1289/ehp.1103781

Figure 3.

Figure 3

BPA did not directly transactivate ER-α in GC-1 and SkBr3 cells, as shown by firefly luciferase activity in GC-1 cells (A) and SkBr3 cells (B–D). (A) GC-1 cells transfected with the ER luciferase reporter plasmid ERE-luc (XETL) and treated with E2 or BPA (each at 10–9 M), with or without ICI. (B–D) SkBr3 cells transfected with ER luciferase reporter gene XETL and ER‑α expression plasmid (B), with Gal4 reporter gene (GK1) and the Gal4 fusion proteins encoding the HBD of ER (GalER‑α; C), and with GK1 and GalER‑β (D) and treated with E2 or BPA (each at 10–9 M), with and without ICI. See “Materials and Methods” for details. Luciferase activity was normalized to Renillaluciferase expression vector (pRL-TK), and the value for vehicle-treated cells was set as 1-fold induction. Values shown (mean ± SE) represent the results of three independent experiments performed in triplicate. *p < 0.05, compared with control. **p < 0.05, compared with E2.