Figure 5. Importin-α silencing does not affect subcellular localization of PB2 or NP.
(A–D) vRNP complexes containing either WSN-PB2-627K-FLAG or WSN-PB2-627E-FLAG were expressed in unsilenced controls (A), importin-α1 (B), -α3 (C), or -α7 (D) silenced 293T cells. Subcellular distribution of PB2 and NP was analyzed by specific staining against PB2-FLAG and NP protein. Mock transfected cells were used as negative controls.
