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. Author manuscript; available in PMC: 2012 Dec 20.
Published in final edited form as: Biochemistry. 2011 Nov 29;50(50):10860–10875. doi: 10.1021/bi201264y

Figure 8. Effects of cysteine mutations and substitutions of SR-BI on HDL-binding activity and selective HDL-CE uptake by CHO cells.

Figure 8

Figure 8

CHO cells were transiently transfected with vector alone, V5-rat SR-BI or the indicated mutants. Thirty-six hours post-transfection, cells were incubated with a culture medium containing 50 μg protein/ml of [125I-DLT]-[3H]COE-labeled hHDL3 for 5h. At the end of incubation, the cells were processed for the determination of [125I-DLT]-hHDL3 binding activity and the quantification of selectively internalized HDL-[3H]COE as described under “Materials and Methods.” The results are Mean ± SE at least three independent experiments and normalized to per unit of total expressed SR-BI. A, selective HDL-CE uptake and B,HDL binding activity.