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. Author manuscript; available in PMC: 2012 Dec 20.
Published in final edited form as: Biochemistry. 2011 Nov 29;50(50):10860–10875. doi: 10.1021/bi201264y

Table 1. List of Primers.

Primer Designation Primer Sequence
C21S-Forward 5′-CTAGGGCTGCTGAGTGCTGCGCTCG-3′
C21S-Reverse 5′-CGAGCGCAGCACTCAGCAGCCCTAG-3′
C251S-Forward 5′-CTATTGGCATTCGGAACAGAGCAACATGATCAATGGTAC-3′
C251S-Reverse 5′-GTACCATTGATCATGTTGCTCTGTTCCGAATGCCAATAG-3′
C280S-Forward 5′-CTTCAGCCCAGAAGCCAGCAGATCTATGAAGCT-3′
C280S-Reverse 5′-AGCTTCATAGATCTGCTGGCTTCTGGGCTGAAG-3′
C321S-Forward 5′-CTAATGAAGGCTTCAGCCCGTGCCGCGAG-3′
C321S-Reverse 5′-CTCGCGGCACGGGCTGAAGCCTTCATTAG-3′
C323S-Forward 5′-GCTTCTGCCCGAGCCGCGAGTCC-3′
C323S-Reverse 5′-GGACTCGCGGCTCGGGCAGAAGC-3′
C334S-Forward 5′-CAGAATGTCAGCACCAGCAGGTTTGGTGCGC-3′
C334S-Reverse 5′-GCGCACCAAACCTGCTGGTGCTGACATTCTG-3′
C384S-Forward 5′-GGGATCCCCATGAACAGTTCCGTGAAGATGC-3′
C384S-Reverse 5′-GCATCTTCACGGAACTGTTCATGGGGATCCC-3′
C470S-Forward 5′-TACCAACTGCGCAGCCAGGAGAAAAGCTTTTTATTTTG-3′
C470S-Reverse 5′-CAAAATAAAAAGCTTTTCTCCTGGCTGCGCAGTTGGTA-3′
V3C-Forward 5′-ACGCGAACATGGGCTGCAGCTCCAGGGCAC-3′
V3C-Reverse 5′-GTGCCCTGGAGCTGCAGCCCATGTTCGCGT-3′
G453C-Forward 5′-YGCTGCTGGGGCTTGGATGCCTCCTGC-3′
G453C-Reverse 5′-GCAGGAGGCATCCAAGCCCCAGCAGCA-3′
Y462C-Forward 5′-GCTTCTGGTGCCCATCATTTGCCAACTGCGCA-3′
Y462C-Reverse 5′-TGCGCAGTTGGCAAATGATGGGCACCAGAAGC-3′