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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Mol Cancer Res. 2011 Nov 15;10(1):25–33. doi: 10.1158/1541-7786.MCR-11-0456

Figure 3.

Figure 3

PELP1 knockdown affects expression of selective genes involved in EMT. A, RNA isolated from MDA-MB231-siRNA negative control and MDA-MB231-PELP1siRNA-expressing cells was hybridized to the human EMT Array. Changes in the gene expression were analyzed by using SABioscience software with actin as a control for normalization. Representative genes downregulated upon PELP1 depletion are shown. B, Real-time qPCR validation of the changes in gene expression in MDA-MB231 cells stably expressing negative control shRNA or PELP1-shRNA. C, MDA-MB231 cells were transiently transfected with control or PELP1-specific siRNA and the expression of indicated genes was analyzed by real-time qPCR. *, p ≤ 0.05, t test.