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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Cell Microbiol. 2011 Nov 3;14(2):226–238. doi: 10.1111/j.1462-5822.2011.01714.x

Fig. 3.

Fig. 3

Dock180 participates in C. jejuni invasion of epithelial cells. HeLa cells were transfected with Dock180 or scrambled (Scr) siRNA. Panels: A) Invasion of the C. jejuni wild-type strain in untreated, Dock180 siRNA-treated, and Scr siRNA-treated cells. Also shown is the number of bacteria internalized for the C. jejuni ciaC mutant. The values represent the mean number of internalized bacteria ± standard deviation. B) Rac1 activation in host cells infected with C. jejuni wild-type strain. Whole cell lysates were processed after 15 minutes of incubation and analyzed for activated Rac1 by G-LISA™. The mean ± standard deviation of total active Rac1 is indicated in Relative Optical Density. The data shown represent at least 10 samples of each condition analyzed in duplicate. The asterisks indicate significance (P < 0.01) in the invasiveness or Rac1 activation of the C. jejuni wild-type strain in untreated versus Dock180-treated cells, as determined using the Student’s t test. C) Whole cell lysates of untreated, Dock180 siRNA-treated, and Scr siRNA-treated INT 407 cells (from left to right) analyzed by immunoblotting with an α-Dock180 antibody.