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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Mol Cancer Res. 2011 Nov 7;10(1):11–24. doi: 10.1158/1541-7786.MCR-11-0256

Figure 4. CX3CL1/CX3CR1-dependent cell migration.

Figure 4

(A) Two EOC cell lines, Caov-3 and SKOV-3, were either not transfected, transfected with control siRNA, or transfected with CX3CR1 siRNA and then subjected to a cell migration assay in a Transwell chamber in the presence or absence of 5 nM CX3CL1 for 5 h. (B) SKOV-3 cells were either not transfected or transiently transfected with control or CX3CR1 siRNAs and then subjected to a Transwell cell migration assay using human peritoneal ascites fluid (specimen #5, respectively, Table 2). Images on the right illustrate the numbers of migrated cells in each condition. Downregulation of CX3CR1 by siRNA in SKOV-3 cells was analyzed by Western blot and quantified by digital densitometry. GAPDH served as a loading control. *p<0.05. (C) Normal ovarian surface epithelial cells (HOSEpiC) and immortalized ovarian surface epithelial cells (T1074) were allowed to migrate for 5 h in the presence or absence of 5 nM CX3CL1 in a Transwell chamber. The number of cells that migrated in the control condition without added CX3CL1 was set as “1”, and other values were calculated relative to this level. These data represent the average of three experiments performed in triplicate. *p<0.05.