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. Author manuscript; available in PMC: 2012 Nov 10.
Published in final edited form as: Oncogene. 2011 Sep 26;31(19):2471–2479. doi: 10.1038/onc.2011.424

Figure 2.

Figure 2

Knockdown of FOXD3 increases cell death after acute treatment with PLX4720. (a) 1205Lu cells were transfected with non-targeting control siRNA or three distinct FOXD3 siRNA sequences (#2, #5, and #18). After 72 hrs, fresh media containing either DMSO or 5 μM PLX4720 was added for an additional 24 hrs. Cell lysates were analyzed by Western blotting, as indicated. (b) Same as (a) except with WM115 cells. (c) Same as (a), except that cells were harvested and stained with Annexin V-APC for cell death analysis after 48 hrs of PLX4720 treatment. Representative traces are shown. X axis, fluorescence intensity; Y axis, cell counts, with percent of Annexin V-APC staining-positive cells indicated. Quantitation of data from three independent experiments is represented by the mean percentage of cells staining positive for Annexin V-APC. Error bars represent standard error. **p value<0.01 comparing PLX4720-treated, non-targeting control knockdown cells to PLX4720-treated FOXD3 knockdown cells based on unpaired Student t-test. (d) Same as (c), except that WM115 cells were analyzed.