Figure 2.

Postreplication Repair Is Specifically Required to Tolerate rNMPs-Containing Chromosomes

Sensitivity to sublethal doses of HU was assayed as described in Figure 1. Pictures were taken after 3 days of incubation. The contribution of NER (A), BER (A), the two branches of PRR (B), and RAD51 (D) was tested. In (C), Quantification of cell survival was obtained as described in Figure 1H. The graph is representative of three independent experiments. Error bars describe standard deviation. It is worth noting that mms2Δ TLSΔ cells, despite being sensitive to HU in the spot tests, do not exhibit increased cell lethality, suggesting that the HU sensitivity derives from a very slow cell-cycle progression. In (E), tetrads derived from a cross between rnh1Δ rnh201Δ and rad52Δ were dissected on YPD plates. Five tetrads (1–5) are shown.The circles on the figure indicate the position of the original rhn1Δ rnh201Δ rad52Δ spores. Cells derived from such microcolonies do not grow when restreaked, revealing that a rad52Δ mutation is synthetic lethal with deletion of the RNH1 and RNH201 genes. TLSΔ comprises rev1Δ rev3Δ rev7Δ rad30Δ.