Fig. 2.

PCR amplification of the foreign genes in the transgenic Chinese cabbage lines. a PCR analysis of 14 putative LL-37 Chinese cabbage lines (T0) using the 35SF (5′-ATGGAGTCAAAGATTCAAATAGAG-3′) and LL-37R (5′-CGAGAGCTCCTAGGACTCTGTCCTGGG-3′) primers. Lanes: M 1 kb ladder, P positive control (pBI LL-37), C non-transformed plant, 1–14 putative transformants. b Four T3 (B21, 22, 23, 24) of single transgenic homozygous lines from T2 (B11, 12, 13, 14) were reconfirmed by PCR after genetic segregation events by kanamycin resistance. Amplification products of the 35S promoter, NptII, and LL-37 were separated using a 1.5% agarose gel. Lanes: M DNA ladder, C non-transformed plant, P pBILL-37, B21–B24 independent T3 transgenic homozygous lines