Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Analysis of the LL-37 gene expression in the transgenic homozygous lines by using reverse transcription polymerase chain reaction. Total RNA was isolated from each plant, and 0.5 μg of this RNA was amplified with LL-37-specific primers. The Chinese cabbage actin gene was amplified as a loading control. The amplification products of the LL-37 gene were separated using a 1.5% agarose gel. Lanes: M DNA ladder, WT wild-type plant, B21–B24 independent transgenic homozygous lines
