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. 1982 Feb 11;10(3):967–977. doi: 10.1093/nar/10.3.967

Inducer-responsive expression of the cloned human interferon beta 1 gene introduced into cultured mouse cells.

S Ohno, T Taniguchi
PMCID: PMC326214  PMID: 6174942

Abstract

A hybrid plasmid was constructed from pSV2-Ecogpt (Mulligan and Berg, 1981) and the cloned 1.8 kilobase chromosomal DNA segment which contains the human interferon (HIFN-beta 1) gene and its flanking sequences. Cultured mouse FM3A cells were transfected by the hybrid plasmid and several Ecogpt+ clones were selected. Southern blot analysis of the DNA from these mouse cell clones showed that two of them contained a few copies of the 1.8 kilobase human DNA. Synthesis of mRNA characteristic of the HIFN-beta 1 mRNA increased as measured by RNA blot analysis when those clones were treated with Newcastle disease virus or with poly(I):poly(C). In addition, the induced mouse cell clones gave rise to the synthesis of human IFN-beta 1. These results suggest the presence of a unique nucleotide sequence in the 1.8 kilobase DNA which is sufficient for the inducer-responsive expression of the human IFN-beta 1 gene.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abreu S. L., Bancroft F. C., Stewart W. E., 2nd Interferon priming. Effects on interferon messenger RNA. J Biol Chem. 1979 May 25;254(10):4114–4118. [PubMed] [Google Scholar]
  2. Breathnach R., Mantei N., Chambon P. Corrected splicing of a chicken ovalbumin gene transcript in mouse L cells. Proc Natl Acad Sci U S A. 1980 Feb;77(2):740–744. doi: 10.1073/pnas.77.2.740. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Buetti E., Diggelmann H. Cloned mouse mammary tumor virus DNA is biologically active in transfected mouse cells and its expression is stimulated by glucocorticoid hormones. Cell. 1981 Feb;23(2):335–345. doi: 10.1016/0092-8674(81)90129-x. [DOI] [PubMed] [Google Scholar]
  4. Degrave W., Derynck R., Tavernier J., Haegeman G., Fiers W. Nucleotide sequence of the chromosomal gene for human fibroblast (beta 1) interferon and of the flanking regions. Gene. 1981 Aug;14(3):137–143. doi: 10.1016/0378-1119(81)90109-8. [DOI] [PubMed] [Google Scholar]
  5. Dierks P., van Ooyen A., Mantei N., Weissmann C. DNA sequences preceding the rabbit beta-globin gene are required for formation in mouse L cells of beta-globin RNA with the correct 5' terminus. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1411–1415. doi: 10.1073/pnas.78.3.1411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Frost E., Williams J. Mapping temperature-sensitive and host-range mutations of adenovirus type 5 by marker rescue. Virology. 1978 Nov;91(1):39–50. doi: 10.1016/0042-6822(78)90353-7. [DOI] [PubMed] [Google Scholar]
  7. Graham F. L., van der Eb A. J. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 1973 Apr;52(2):456–467. doi: 10.1016/0042-6822(73)90341-3. [DOI] [PubMed] [Google Scholar]
  8. Gross-Bellard M., Oudet P., Chambon P. Isolation of high-molecular-weight DNA from mammalian cells. Eur J Biochem. 1973 Jul 2;36(1):32–38. doi: 10.1111/j.1432-1033.1973.tb02881.x. [DOI] [PubMed] [Google Scholar]
  9. Gross G., Mayr U., Bruns W., Grosveld F., Dahl H. M., Collins J. The structure of a thirty-six kilobase region of the human chromosome including the fibroblast interferon gene IFN-beta. Nucleic Acids Res. 1981 Jun 11;9(11):2495–2507. doi: 10.1093/nar/9.11.2495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hamer D. H., Kaehler M., Leder P. A mouse globin gene promoter is functional in SV40. Cell. 1980 Oct;21(3):697–708. doi: 10.1016/0092-8674(80)90433-x. [DOI] [PubMed] [Google Scholar]
  11. Houghton M., Jackson I. J., Porter A. G., Doel S. M., Catlin G. H., Barber C., Carey N. H. The absence of introns within a human fibroblast interferon gene. Nucleic Acids Res. 1981 Jan 24;9(2):247–266. doi: 10.1093/nar/9.2.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hynes N. E., Kennedy N., Rahmsdorf U., Groner B. Hormone-responsive expression of an endogenous proviral gene of mouse mammary tumor virus after molecular cloning and gene transfer into cultured cells. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2038–2042. doi: 10.1073/pnas.78.4.2038. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kataoka T., Yamawaki-Kataoka Y., Yamagishi H., Honjo T. Cloning immunoglobulin gamma 2b chain gene of mouse: characterization and partial sequence determination. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4240–4244. doi: 10.1073/pnas.76.9.4240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kuroki T. Colony formation of mammalian cells on agar plates and its application to Lederberg's replica plating. Exp Cell Res. 1973 Jul;80(1):55–62. doi: 10.1016/0014-4827(73)90274-7. [DOI] [PubMed] [Google Scholar]
  15. Kurtz D. T. Hormonal inducibility of rat alpha 2u globulin genes in transfected mouse cells. Nature. 1981 Jun 25;291(5817):629–631. doi: 10.1038/291629a0. [DOI] [PubMed] [Google Scholar]
  16. Lai E. C., Woo S. L., Bordelon-Riser M. E., Fraser T. H., O'Malley B. W. Ovalbumin is synthesized in mouse cells transformed with the natural chicken ovalbumin gene. Proc Natl Acad Sci U S A. 1980 Jan;77(1):244–248. doi: 10.1073/pnas.77.1.244. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lawn R. M., Adelman J., Franke A. E., Houck C. M., Gross M., Najarian R., Goeddel D. V. Human fibroblast interferon gene lacks introns. Nucleic Acids Res. 1981 Mar 11;9(5):1045–1052. doi: 10.1093/nar/9.5.1045. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mantei N., Boll W., Weissmann C. Rabbit beta-globin mRNA production in mouse L cells transformed with cloned rabbit beta-globin chromosomal DNA. Nature. 1979 Sep 6;281(5726):40–46. doi: 10.1038/281040a0. [DOI] [PubMed] [Google Scholar]
  19. McMaster G. K., Carmichael G. G. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835–4838. doi: 10.1073/pnas.74.11.4835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mulligan R. C., Berg P. Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2072–2076. doi: 10.1073/pnas.78.4.2072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Nakano N. Establishment of cell lines in vitro from a mammary ascites tumor of mouse and biological properties of the established lines in a serum containing medium. Tohoku J Exp Med. 1966 Jan 25;88(1):69–84. doi: 10.1620/tjem.88.69. [DOI] [PubMed] [Google Scholar]
  22. Ohno S., Taniguchi T. Structure of a chromosomal gene for human interferon beta. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5305–5309. doi: 10.1073/pnas.78.9.5305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Scangos G., Ruddle F. H. Mechanisms and applications of DNA-mediated gene transfer in mammalian cells - a review. Gene. 1981 Jun-Jul;14(1-2):1–10. doi: 10.1016/0378-1119(81)90143-8. [DOI] [PubMed] [Google Scholar]
  24. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  25. Taniguchi T., Mantei N., Schwarzstein M., Nagata S., Muramatsu M., Weissmann C. Human leukocyte and fibroblast interferons are structurally related. Nature. 1980 Jun 19;285(5766):547–549. doi: 10.1038/285547a0. [DOI] [PubMed] [Google Scholar]
  26. Taniguchi T., Pang R. H., Yip Y. K., Henriksen D., Vilcek J. Partial characterization of gamma (immune) interferon mRNA extracted from human lymphocytes. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3469–3472. doi: 10.1073/pnas.78.6.3469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. de Maeyer-Guignard J., de Maeyer E., Montagnier L. Interferon messenger RNA: translation in heterologous cells. Proc Natl Acad Sci U S A. 1972 May;69(5):1203–1207. doi: 10.1073/pnas.69.5.1203. [DOI] [PMC free article] [PubMed] [Google Scholar]

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