Architecture of rat heart visualized from DT-MRI with 0.2 mm isotropic voxels. (a,b) Transmural rotational anisotropy, visualized by a local average cell angle of inclination, or helix angle, estimated from the primary eigenvector. (c) ‘Sheet’ angle estimated from the secondary and tertiary eigenvectors. (d) Fractional anisotropy, computed from eigenvalues at each voxel by equation (2.1).