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Optical mapping of surface spatio-temporal activity. (a) Epi-fluorescence optical mapping of Langendorff perfused heart. (b) Simulation of the subsurface tissue contribution to the surface optical signal for Di-4-ANEPPS, from a rotationally anisotropic transmural slab of depth 5 mm and radius 10 mm. Scale bar indicates the percentage contribution of each pixel to the total signal recorded from the top left pixel on the epicardial surface.
