Effect of chronic FLX treatment on a) PDE and AC gene expression and b) cAMP levels in the rat pineal gland. Gene expression results are expressed as mean ± SEM for FLX as percentage of saline-treated control (SAL) (n = 9–10 animals/group). Cyclic AMP (cAMP) enzyme immunoassay results are expressed as mean ± SEM concentrations of cAMP (in pmol/mL) for SAL and FLX.
Note: Asterisks indicate significant differences (**p < 0.01, ***p < 0.001) using Student’s t-test.
Abbreviations: AC, adenylate cyclases; AMP, adenosine monophosphate; FLX, fluoxetine; SAL; saline-treated control; PDE, phosphodiesterase; SEM, standard error of mean.