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. 2010 Apr 1;2:31–40. doi: 10.2147/CPAA.S6262

Figure 1.

Figure 1

Genes for both NMDAR1 and NMDAR2B receptors are expressed in cultured small-cell lung cancer cells. A) The expression of NMDAR1 receptors in the cultured cell lines by RT-PCR using primers designed to amplify different regions of the coding sequence (see text for details). Shown are results obtained for classical cell lines NCI H345, NCI H146, and DMS 53, and variant cell line NCI H82. B) The expression of NMDAR2B receptors by cultured cell lines by RT-PCR using primers designed to amplify different regions of the coding sequence for this receptor (see text for details). Shown are results obtained for classical cell lines NCI 146 and DMS 53, and variant cell line NCI H82. Sequence analysis of the products using nested primers indicated normal NMDAR1 and NMDAR2B messages. Products were separated on 1.5% agarose gels, which were then stained with ethidium bromide. In each case one band of the expected size was obtained for the amplification product.