Figure 5.

Vγ1⁺ γδ T cells acquire upregulatory activity as well after in vitro activation. (A) Preparation of highly enriched γδ T cells expressing Vγ1 or Vγ4 TCR segments. Vγ1⁺ T cells were isolated from mice expressing a Vγ1 TCR transgene,¹⁴ and unseparated γδT cells isolated from immunized B6 mice dominantly express Vγ4⁺.²⁴ (B) Intracellular staining assay. Activated and resting Vγ4⁺ or Vγ1⁺ γδ T cells were prepared as detailed in Materials and Methods. Experimental procedures were the same as those described in the legend to Figure 2. (C, D) Assessment of total T cell numbers and IL-17⁺ (Th17 polarized conditions) or IFN-γ⁺ (nonpolarized conditions) T cells after 5 days of in vitro stimulation. These studies were repeated four times. **P ≤ 0.01; differences were considered very significant.