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Immunodetection of Clara-cell secretory protein (CCSP) in K14-rtTA+/0/TRE-Cre+/0/DE3+/+ (BiTg) mice. CCSP staining of tracheal (A), bronchial (B), and terminal bronchiolar (C) epithelia. Images are representative of large, highly autofluorescent Clara-type cells. CCSP, red; 4',6-diamidino-2-phenylindole (DAPI), blue. K14, keratin 14; rtTA, reverse-tetracycline transactivator; TRE, tetracycline-responsive element; DE3, β-catenin floxed exon 3.
