TABLE 1.
ANTIBODIES AND PRIMERS USED IN THE STUDY
| Antigen | Source | Concentration Used | Species, Isotype |
| Calponin | Abcam Ab700 | 1:400 | Mouse IgG |
| Smooth muscle actin | Sigma 2547 | 1:1,000 | Mouse IgG |
| pERK/1-2 | Cell Signaling 4377S | 1:00 IF, 1:1,000 blot | Rabbit IgG |
| ERK/1-2 | Cell Signaling 4695 | 1:1,000 | Rabbot IgG |
| ATF6 | Imgenex 273 | 1:100 IF, 1:250 blot | Mouse IgG |
| Beta-action | Sigma A5316 | 1:1,000 | Mouse IgG |
| peIF2alpha | Cell Signaling 3597 | 1:50 IF, 1:500 blot | Rabbit IgG |
| Total eIF2alpha | Cell Signaling 2103 | 1:1,000 | Mouse IgG |
| Hyaluronic acide–binding protein, biotinylated | Seigaku | 20 ng/ml | N/A |
| Streptavidin–Alexa 488 | Invitrogen S11223 | 1:500 | N/A |
| Anti-mouse Cy3 | Chemicon AP124C | 1:500 | Goat |
| Anti-rabbit Alexa 488 | Invitrogen A11034 | 1:500 | Goat |
| Gene | Forward Primer | Reverse Primer | |
| sXBP-1 | GACTCCGCAGCAGGTG | GCGTCAGAATCCATGGGA | |
| XBP-1 | CAGACTACGTGCGCCTCTGC | CTTCTGGGTAGACCTCTGGG | |
| HPRT | AAGCTTGCTGGTGAAAAGGA | CAAGGGCATATCCAACAACA | |
| Hyal2 | ACAGAACTTAGCCAGATGGA | GGTGAGAGTCATAGCTACCA | |
| HAS2 | AGGGCCTGCCAGTCTTATTT | CCCTGTTGGTAAGGTGCCTA |