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Western blot analyses of effects of TM5275 on the expression of collagen, α-SMA, and TGF-β proteins in lungs of TGF-β1–treated mice. Top, representative Western blotting images of collagen, α-SMA, and TGF-β. β-actin was used to show equal protein loading. Bottom, summaries of semiquantified data of collagen, α-SMA, and TGF-β band densities relative to β-actin, as determined by Image J software. *Significantly different from saline + solvent–treated control group (P < 0.05, n = 4–5).
