Figure 6.

Effects of TM5275 on TGF-β1–induced expression of α-SMA, fibronectin, and PAI-1 in human lung fibroblasts. (A) Human lung fibroblasts (CCL-210 cells) were treated with 1 ng/ml of TGF-β1 in the presence or absence of 75 μM of TM5275 for 24 hours. (B) CCL-210 cells were treated with 1 ng/nl of TGF-β1 for 1 or 6 hours. (C) CCL-210 cells were pretreated with 1 ng/ml of TGF-β1 for 6 hours, and then with 75 μM of TM5275 for 24 hours. Top, representative Western blotting images. Bottom, summaries of the semiquantified data of band densities relative to β-actin band densities. ^aSignificantly different from solvent alone group. ^bSignificantly different from TGF-β1 alone–treated group (P < 0.05, n = 6).