Figure 3.
Deletion of Nrf2 in Clara cells exacerbates hyperoxia-induced lung inflammation. Nrf2f/f mice and Nrf2∆cc mice were exposed to room air or hyperoxia for 60 hours. BAL fluid was collected from the right lung, centrifuged, and stained with a Diff-Quik stain kit. Differential cell counts were performed to analyze total cells (A), macrophages (B), epithelial cells (C), and neutrophils (D), and are expressed as means ± SEM. One-way ANOVA, followed by Newman-Keuls post hoc analysis, was performed for multiple group comparisons, whereas two-way ANOVA with a Bonferroni correction was used to compare interactions between genotype and hyperoxia. In both cases, P ≤ 0.05 was considered statistically significant. *Room air versus hyperoxia. †Nrf2f/f versus Nrf2∆cc counterparts. Open bars, room-air controls; solid bars, hyperoxia.